Publications
All the publications:
PubMed search for C. Saveanu →, for papers as first author→ or last author→ (links open in new tab).
Selected publications (or preprints)
Audebert L, Feuerbach F, Zedan M, Schürch AP, Decourty L, Namane A, Permal E, Weis K, Badis G, Saveanu C* (2024) RNA degradation triggered by decapping is largely independent of initial deadenylation. EMBO J.PMID: 39233754
Biology manuals describe mRNA degradation as following an obligatory 1) deadenylation and 2) decapping sequence of molecular events. Careful examination of this assertion led to a different view about the life of mRNA in S. cerevisiae. For most unstable mRNAs, it turns out that deadenylation is not required for initiating mRNA degradation. However, we also identified situations in which deadenylation, rather than decapping plays a role in mRNA degradation. This work has been done in collaboration with the group of Karsten Weis at ETH Zürich, where the SLAM-Seq results have been obtained.
Inhibition of deadenylation by rapid degradation (degron-induced) of the two deadenylase components of the CCR4-NOT complex leads to longer poly(A) tails for a reporter mRNA, even if the half-life of the reporter was not affected.
Gilbert A & Saveanu C* (2022) Unusual SMG suspects recruit degradation enzymes in nonsense-mediated mRNA decay. Bioessays e2100296. - review PMID: 35266563
In this review, we discuss the challenges in understanding mRNA degradation. Among them, the heterogeneity of the molecules of RNA, which are a mix of old and new, polyadenylated and deadenylated, full length and fragments. We also take a look at the conservation of the factors involved. Our previous work and recent collaborations with H. Le Hir's and M. Graille's laboratory clearly identified the equivalence of yeast and metazoan NMD co-factors. As an example, NMD4 retained the PIN domain typical of SMG-6 together with a small domain of interaction with Upf1. A specific SMG-6 motif identified by the work of the Graille team is important for the interaction of human UPF1 with SMG-6.
BioS/B/Y refers to proximity biotinylation studies and AffH/L/Br refers to large scale co-purification results.
Namane A & Saveanu C* (2022) Composition and dynamics of protein complexes measured by quantitative mass spectrometry of affinity purified samples. book chapter in Methods in Molecular Biology 2477:225-236, Yeast functional genomics, (Springer), preprint at HAL pasteur-03384570v1 . PMID: 35524120 ISBN: 978-1-0716-2257-5
This methods article describes in detail the use of the ZZ motif derived from the S. aureus protein A to purify protein complexes from yeast (S. cerevisiae). It also includes the recipes for the preparation of IgG-magnetic beads and hints about mass spectrometry results analysis.
Protein enrichment is on a log2 scale, in comparison with total protein abundance. The interaction of Upf2, Upf3, and decapping complex components depend on the CH region of yeast Upf1
Decourty L, Malabat C, Frachon E, Jacquier A & Saveanu C*. Investigation of RNA metabolism through large-scale genetic interaction profiling in yeast. Nucleic Acids Research (2021), Sep 7;49(15):8535-8555. PMID: 34358317
The results can be explored through an interactive web interface at : hub05.hosting.pasteur.fr/GIM_interactions/→
Ribosomal protein gene deletion led to growth phenotypes that were either alleviated by the absence of PUF4 or aggravated by it. In the case of RPL9A deletion, the suppression is due to the inhibitory effect of Puf4 on RPL9B expression.
Dehecq M, Decourty L, Namane A, Proux C, Kanaan J, Le Hir H, Jacquier A & Saveanu C*. Nonsense-mediated mRNA decay involves two distinct Upf1-bound complexes. EMBO J (2018), Nov 2;37(21):e99278. PMID: 30275269
The protein association results can be explored through an interactive web interface at : hub05.hosting.pasteur.fr/NMD_complexes/→
A little more than 100 purification experiments under various conditions identified proteins that are mutually exclusive in their association with Upf1.
Decourty L, Doyen A, Malabat C, Frachon E, Rispal D, Séraphin B, Feuerbach F, Jacquier A & Saveanu C*. Long open reading frame transcripts escape nonsense-mediated mRNA decay in yeast. Cell Rep (2014), Feb 27;;6(4):593-8. PMID: 24529707
A multiplexed assay for over 800 NMD reporters identified coding sequence length as a major determinant of mRNA stability.
Degradation of reporter mRNA was followed over time after specifically blocking their transcription. Longer coding sequences were protective against mRNA degradation through NMD.